Western Blot Substrates and Substrate Kits
Abcam ECL Substrate Kit (High Sensitivity), 200ML
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.
The product is subject to the following: Abcam Restricted Use Statement
Cell Signaling Technology SignalFire™ ECL Reagent, Chemiluminescent Substrate, Western Blot, 50 mL
SignalFire™ ECL Reagent is an enhanced chemiluminescent substrate designed for the detection of picogram amounts of protein via western blot analysis. By utilizing horseradish peroxidase (HRP) to convert luminol in the presence of hydrogen peroxide, this reagent produces an excited intermediate dianion that emits light upon returning to its ground state. Compared to standard substrates, this system offers enhanced signal robustness and an extended output duration of up to 3 hours.
- Provides high sensitivity for detection of picogram levels of protein
- Features extended signal output for up to 3 hours
- Compatible with HRP-conjugated secondary antibodies
- Requires no further water dilution due to 2X concentrate formulation
- Stable for 36 months when stored at room temperature
Revvity Health Sciences Inc TopSeal-S 96-well, clear, case of 100
TopSeal-S 96-well, clear, case of 100
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
National Diagnostics ProtoGlow ECL | 200mL
ProtoGlow ECL delivers the latest technology developed for enhanced chemiluminescent detection on Western blots. The unique chemistry of the ProtoGlow ECL system increases the sensitivity of Western blots by up to 20 fold. This allows the detection of proteins at lower abundance and/or the use of higher dilutions of primary and secondary antibodies, economizing on these expensive reagents.
Genetex Inc Trident femto Western HRP Substrate
GeneTex Trident femto Western HRP Substrate can detect horseradish peroxidase at the femtogram level. The substrate is supplied as two components.
Seracare Life Sciences Inc TMB 1-C MEMBRANE HRP SUBSTRATE
Most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. 1-component format of 3, 3', 5, 5' - Tetramethylbenzidine, no reagent preparation, hence, very convenient.
LICORbio WesternSure PREMIUM Chemiluminescent Substrate (100 mL kit)
WesternSure PREMIUM Chemiluminescent Substrate is a highly sensitive, enhanced substrate for detecting horseradish peroxidase (HRP) on immunoblots. It has sensitivity comparable with SuperSignal West Dura for greater than 60 minutes post-incubation, it has the most intense signal with longest duration, and it is ideal for use with digital imaging systems. The substrate pack contains Luminol Enhancer Solution and Stable Peroxidase Solution.