Western Blot Substrates and Substrate Kits
Revvity Health Sciences Inc Western Lightning Plus, Chemiluminescent Substrate (680 mL)
Western Lightning Plus, Chemiluminescent Substrate (680 mL)
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Bio Rad Laboratories PRECISION PLUS PROTEIN UNSTAIN
Standards, Unstained; Bio-Rad; Precision Plus Protein; 1mL; 100 applications
AdipoGen BCIP p-toluidine salt
Chemical. CAS 6578-06-9. Formula C8H6BrClNO4P . C7H9N. MW 433.62. BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry IHC or Western blot WB.
Abcam ECL Substrate Kit (Ultra High Sensitivity), 100ML
Ultra High Sensitivity ECL Substrate Kit ab133409 is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.
The product is subject to the following: Abcam Restricted Use Statement
Rockland Immunochemicals BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE - NBT-100
BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
Sigma Aldrich Fine Chemicals Biosciences Atto 488 Protein Labeling
This kit contains sufficient amounts of reactive dye buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST BCIP/NBT
Synonym: BCIP/NBT Alkaline Phosphatase Substrate. Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.